Journal: Biochemical and biophysical research communications

Article Title: EP3 activation facilitates bladder excitability via HCN channels on ICCs.

doi: 10.1016/j.bbrc.2017.01.131

Figure Lengend Snippet: Fig. 2. Effect of EP3 on the expression and function of HCN channels. (A, D) Western blot analysis compared the expression of HCN channels between mouse bladders treated by intravesical instillation with sulprostone and mouse bladders treated with NS. GAPDH was used as the control (n ¼ 8). (B, C) Whole-cell patch clamping was used to determine the effects of PGE2 and sulprostone on the HCN channel current (n ¼ 6). (E) Single-cell PCR showing that the cells used for patch clamping were positive for the ICC marker c-Kit. *p < 0.05, **p < 0.01.

Article Snippet: PGE2 (10 6 M; Sigma, USA) and sulprostone (10 5 M; Santa Cruz, USA) were separately added to the bath.

Techniques: Expressing, Western Blot, Control, Marker

Journal: Biochemical and biophysical research communications

Article Title: EP3 activation facilitates bladder excitability via HCN channels on ICCs.

doi: 10.1016/j.bbrc.2017.01.131

Figure Lengend Snippet: Fig. 3. Effect of EP3 on the intracellular calcium of ICCs. (A, B) Nine images were chosen to represent the change in concentration of intracellular calcium in the ICCs after the agonist of EP3 and antagonist of HCN channels were added. (C, D, E, F) Real-time relative fluorescence intensities (RFI, F/F0) were depicted as continuous plots. PGE2, sulprostone and ZD7288 were applied to test their effects on the concentration of intracellular calcium in ICCs (n ¼ 8). *p < 0.05, **p < 0.01.

Article Snippet: PGE2 (10 6 M; Sigma, USA) and sulprostone (10 5 M; Santa Cruz, USA) were separately added to the bath.

Techniques: Concentration Assay

Journal: Biochemical and biophysical research communications

Article Title: EP3 activation facilitates bladder excitability via HCN channels on ICCs.

doi: 10.1016/j.bbrc.2017.01.131

Figure Lengend Snippet: Fig. 4. Effect of EP3 on the contraction of bladder detrusor strips. (A) Bladder detrusor strip test showing the effects of PGE2, sulprostone and ZD7288 on the amplitude of wild- type mouse bladder detrusors (n ¼ 8). (B) Effects of PGE2 and sulprostone on the amplitude of the HCN1/ mice bladder detrusor (n ¼ 8). (C) Effects of ZD7288, PGE2 and sulprostone on the amplitude of the rat bladder detrusor (n ¼ 8). (D) Bladder detrusor strip test revealing the effects of L798106 (an antagonist of EP3) and PGE2 on the amplitude of rat bladder detrusor (n ¼ 8). (E, F) Graphs showing the different effects of PGE2 and sulprostone on wild-type mouse bladders and HCN1/ mice bladders (n ¼ 8). *p < 0.05, **p < 0.01.

Article Snippet: PGE2 (10 6 M; Sigma, USA) and sulprostone (10 5 M; Santa Cruz, USA) were separately added to the bath.

Techniques: Stripping Membranes

Journal: BMC Pharmacology

Article Title: Cloning and expression of the rabbit prostaglandin EP2 receptor

doi: 10.1186/1471-2210-2-14

Figure Lengend Snippet: Competition binding profile of the EP 2 receptor expressed in COS-1 cells. Membranes were incubated with 1.5–2 nM [ 3 H]PGE 2 and varying concentrations of competitors. The data shown are from a single experiment performed in triplicate and are representative of three to four experiments. Panel A: ■ PGE 2 ; ▼ PGF 2a ; ▲ PGD 2 ; ◆ iloprost. Panel B: □ butaprost free acid; ?? AH13205; ▽ misoprostol; ◇ sulprostone.

Article Snippet: Sulprostone, misoprostol, iloprost and AH13205 were kindly provided by Dr. Harold Kluender of Miles Laboratories, P.J.

Techniques: Binding Assay, Incubation

Journal: The Cochrane Database of Systematic Reviews

Article Title: Uterotonic agents for preventing postpartum haemorrhage: a network meta‐analysis

doi: 10.1002/14651858.CD011689.pub3

Figure Lengend Snippet: Poeschmann 1991

Article Snippet: Funding source , Unclear risk , Sulprostone was supplied by Schering without charge but no other funding sources are reported..

Techniques: Sequencing, Selection

Journal: The Cochrane Database of Systematic Reviews

Article Title: Uterotonic agents for preventing postpartum haemorrhage: a network meta‐analysis

doi: 10.1002/14651858.CD011689.pub3

Figure Lengend Snippet: Van Selm 1995

Article Snippet: Funding source , Unclear risk , Sulprostone was supplied by Schering without charge but no other funding sources are reported..

Techniques: Coagulation, Sequencing, Selection, Staining

Journal: The Cochrane Database of Systematic Reviews

Article Title: Uterotonic agents for preventing postpartum haemorrhage: a network meta‐analysis

doi: 10.1002/14651858.CD011689.pub3

Figure Lengend Snippet: Norchi 1988

Article Snippet: Funding source , Unclear risk , Sulprostone was supplied by Schering without charge but no other funding sources are reported..

Techniques:

Journal: Molecules (Basel, Switzerland)

Article Title: Anti-Periodontitis Effects of Dendropanax morbiferus H.Lév Leaf Extract on Ligature-Induced Periodontitis in Rats.

doi: 10.3390/molecules28020849

Figure Lengend Snippet: Figure 3. Effects of DMWE on LPS-induced NO (□) and PGE2 (■) production in RAW 264.7 cells. LPS, liposaccharide; DMWE, hot-water extracts of DML. Different superscript letters (a, b, c, d) indicate statistical significance in nitric oxide (NO) production (p < 0.05). Different superscript letters (p, q, r, s) indicate statistical significance in prostaglandin E2 (PGE2) production (p < 0.05).

Article Snippet: Molecules 2023, 28, x FOR PEER REVIEW 12 of 16 linked immunosorbent assay (ELISA) PGE2 kit (R&D Systems Inc., Minneapolis, MN, USA).

Techniques: